Analysis of microsatellite markers in medieval cattle remains from central Italy

icaz_paper_icon.jpg

All Titles


  • Analysis of microsatellite markers in medieval cattle remains from central Italy

Dublin Core

Title


Analysis of microsatellite markers in medieval cattle remains from central Italy

Subject

S5-3, Archaeozoology and palaeogenetics: potentials and limits, poster

Description

Abstract:

The study of DNA from archaeological remains offers a valuable tool to interpret the history of ancient animal populations. However, some technical problems makes the analysis of ancient DNA very difficult.

The objectives of this study are: identifying suitable microsatellites markers that can be used in ancient livestock remains; performing a comparative analysis between ancient cattle remains and extant breeds.

Four microsatellites (hel1, csrm60, inra005, hel9) with an allelic length shorter than 160bp, so suitable for degraded DNA, were selected from FAO list (www.faostat.fao.org) and amplified on medieval cattle. DNA was extracted from cattle bones from Ferento, an archaeological site near Viterbo, central Italy. The microsatellites were PCR amplified and analyzed using a CEQ 8800 sequencer. A successful amplification for samples was obtained using 3 microsatellites (hel1, csrm60, inra005).

Allelic data from these three markers were compared with those obtained from 72 European cattle breeds. The genetic relationships between modern and ancient cattle were investigated using a Bayesian clustering approach and a principal component analysis. Our preliminary data cluster Ferento samples together with some extant Italian breeds.

Our results indicate that microsatellite markers may be useful for the study of ancient animal populations.

Next step will be to analyze a larger number of microsatellites to better understand the relationship between ancient and extant cattle breeds.

Authors:

GARGANI Maria1, PARISET Lorraine1, DE MINICIS Elisabetta2 and VALENTINI Alessio1

Affiliations

1 Dipartimento di Produzioni Animali;, Facoltà di Agraria, Università degli Studi della Tuscia, 01100 Viterbo Italy, maria.gargani@unitus.it, pariset@unitus.it, alessio@unitus.it
2, Dipartimento di Scienze del Mondo Antico, Facoltà di Conservazione dei Beni Culturali, Università degli Studi della Tuscia, 01100Viterbo Italy, e.deminicis@unitus.it

Creator

Gargani, Maria
Pariset, Lorraine
De Minicis, Elisabetta
Valentini, Alessio

Date

August 2010

Contribution Form

Document Item Type Metadata

Citation

Gargani, Maria, Pariset, Lorraine, De Minicis, Elisabetta and Valentini, Alessio. " Analysis of microsatellite markers in medieval cattle remains from central Italy ," in BoneCommons, Item #1155, http://alexandriaarchive.org/bonecommons/items/show/1155 (accessed September 24, 2020).