Remarks on diachronic biodiversity in Sardinian sheep by means of mitochondrial DNA analysis

icaz_paper_icon.jpg

All Titles


  • Remarks on diachronic biodiversity in Sardinian sheep by means of mitochondrial DNA analysis

Dublin Core

Title


Remarks on diachronic biodiversity in Sardinian sheep by means of mitochondrial DNA analysis

Subject

S5-2, S6-3, General session, poster

Description

Abstract:

As in other Mediterranean islands, domestic species were imported to Sardinia during different prehistoric and protohistoric Ages. Aimed at evaluating diachronic biodiversity, remains from Sardinian sheep dug out in the last decades have been analyzed. The material consisted in 24 bones and 24 teeth coming from 8 different archaeological sites of various Sardinian regions dating back from late Neolithic to Medieval Age. Ancient DNA was extracted, quantified through spectrometry and separated electrophoretically on agarose gel, and its preservation conditions underwent careful evaluation. Notwithstanding the fact that dental tissues are very poor in cells and mitochondria, DNA amount was about five times higher in teeth than in bones depending on soil pH. Furthermore, ancient DNA from a 176 bp region of the mitochondrial D-loop was PCR-amplified with specific primers and sequenced. The nucleotide sequences obtained were compared against what reported by Genbank and against the sequence obtained from subjects belonging to the modern Sardinian breed.

In all ancient samples a single-base is lacking when compared with the modern sheep. As to the nucleotide differences among ancient sheep, a single-base insertion was detected in the Roman and Medieval sheep in comparison with the Nuragic sheep. Such results, as well as the others in progress will hopefully let us draw the genetic tree of Sardinian sheep breed.

Research financed by P5a grant from Sardinian Region.

Authors:

ZEDDA Marco, BALZANO Francesca, MURA Arcadia, MURA Emilio, GADAU Sergio, LEPORE Gianluca, FARINA Vittorio

Affiliations

Department of Animal Biology, University of Sassari, via Vienna 2, 07100 Sassari, Italy, mzedda@uniss.it

Creator

Zedda, Marco
Balzano, Francesca
Mura, Arcadia
Mura, Emilio
Gadau, Sergio
Lepore, Gianluca
Farina, Vittorio

Date

August 2010

Contribution Form

Document Item Type Metadata

Citation

Zedda, Marco, Balzano, Francesca, Mura, Arcadia, Mura, Emilio, Gadau, Sergio, Lepore, Gianluca and Farina, Vittorio. " Remarks on diachronic biodiversity in Sardinian sheep by means of mitochondrial DNA analysis ," in BoneCommons, Item #1668, http://alexandriaarchive.org/bonecommons/items/show/1668 (accessed August 7, 2020).